# Weissman Lab (Jonathan Weissman)

**Type:** research group
**PI:** Jonathan S. Weissman
**Affiliation:** MIT / Whitehead Institute (formerly UCSF)
**Focus:** Ribosome biology, protein folding, CRISPR functional genomics, Perturb-seq

## Description

The lab that invented and scaled **Perturb-seq** — the single-cell CRISPR screening technology that underpins most of the perturbation biology field. Norman 2019 and Replogle 2022 both came from this lab.

## Key contributions to perturbation biology

- Original Perturb-seq concept (Dixit et al, Cell 2016 — first paper combining CRISPR KO with scRNA-seq)
- Norman 2019 — combinatorial CRISPRa screen, K562
- Replogle 2022 — genome-scale CRISPRi, 2.5M cells

## Connections

- [../papers/norman-2019.md](../papers/norman-2019.md)
- [../papers/replogle-2022.md](../papers/replogle-2022.md)
- [../concepts/perturbation-biology.md](../concepts/perturbation-biology.md)